Glycosylation-dependent opsonophagocytic activity of staphylococcal protein A antibodies
نویسندگان
چکیده
منابع مشابه
Complement-dependent Platelet Injury by Staphylococcal Protein A
A new example of complement-mediated platelet injury has been described. Staphylococcal protein A (SPA) causes rabbit platelet injury as manifested by release of platelet 5-hydroxytryptamine (5HT). This reaction is complement-dependent and occurs over a very small range of SPA concentration, larger amounts being inhibitory. Complement fixation by SPA demonstrates the same narrow SPA concentrati...
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Methods to identify intact multi-protein complexes can help to achieve more effective formulations but their application to OMVs can be difficult due to the hydrophobicity of membrane proteins. Our previous studies of the meningococcal porin complexes using blue native polyacrylamide gel electrophoresis (BNE) (1) allowed the detection of a 700 kDa major complex. Analysis by LC/MS-MS revealed th...
متن کاملThe use and limitation of labeled staphylococcal protein A for study of antineutrophil antibodies.
Antineutrophil antibodies can be detected following their attachment to neutrophils by employing labeled staphylococcal protein A (SPA). Radiolabeled SPA provides a sensitive means for identifying the presence of IgG restricted to subclasses IgG1, IgG2, and IgG4 that will specifically bind to neutrophils and that are found in the serum of patients with isoimmune and autoimmune neutropenia. Howe...
متن کاملRadioimmunoassay for quantitation of antibodies to alphaviruses with staphylococcal protein A.
A radioimmunoassay (RIA) procedure is described for measuring antibodies to alphaviruses in human and other mammalian sera. The test employed protein Abearing Staphylococcus aureus as a solid-phase immunoadsorbent for (3)H-labeled viruses complexed with immunoglobulin G. Using antibodies produced in humans and guinea pigs, the RIA procedure clearly differentiated among antibodies to Venezuelan,...
متن کاملN-Glycosylation of Carnosinase Influences Protein Secretion and Enzyme Activity
OBJECTIVE The (CTG)(n) polymorphism in the serum carnosinase (CN-1) gene affects CN-1 secretion. Since CN-1 is heavily glycosylated and glycosylation might influence protein secretion as well, we tested the role of N-glycosylation for CN-1 secretion and enzyme activity. We also tested whether CN-1 secretion is changed under hyperglycemic conditions. RESULTS N-glycosylation of CN-1 was either ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2020
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.2003621117